protein sequencing
英 [ˈprəʊtiːn ˈsiːkwənsɪŋ]
美 [ˈproʊtiːn ˈsiːkwənsɪŋ]
网络 蛋白质测序; 蛋白质序列测定; 蛋白质顺序分析
双语例句
- Methods: By using automatic analyzer for protein sequencing.
方法：自动蛋白测序仪法。 - All of the nine exons and intron-exon boundaries of protein C gene of the propositus were analyzed by direct sequencing of the corresponding amplified PCR products in DNA from the propositus.
用PCR法对先证者PC基因的9个外显子及其侧翼、内含子2序列进行扩增，PCR产物纯化后直接测序，检测其基因突变。 - Results: The two genes of E protein and GFP were fused in codon frame by DNA sequencing. The fluorescence microscopy showed that the E protein was expressed in the cytoplasm and cell membrane of BHK-21 cells.
结果：经酶切和DNA序列测定鉴定，E蛋白基因正确插入到增强绿荧光蛋白EGFP基因上游，荧光显微镜显示E蛋白表达于细胞浆内，包膜上也有分布。 - Methods PCR based in vitro synthesized protein test ( IVSP) assay and sequencing analysis were used to confirm somatic mutations of whole APC gene in 19 HNPCC patients.
方法采用体外蛋白合成试验和序列分析确定19例HNPCC病例APC体细胞突变。 - Techniques of protein sequencing at the turn of the centuries
世纪之交的蛋白质序列测定技术 - Methods: The cDNA of N-terminal fragment gene of bactericidal/ permeability-increasing protein was amplified by RT-PCR using RNA isolated from human polymorphonuclear leukocytes cells ( PMNL) and confirmed by sequencing.
方法：采用RTPCR技术，从人外周血中性粒细胞的总RNA中扩增BPI蛋白N端片段cDNA编码区基因，DNA序列分析鉴定； - Recent developments in analytical methods for protein characterization, and the growing rate at which whole genome sequencing projects are being completed, have combined to support the emergence and development of the new field of 'proteomics'.
蛋白质鉴定技术的发展与基因组测序计划的实施与相继完成，使得蛋白质组学这一全新的研究领域得以诞生和发展。 - In order to identify these putative proteins, further analysis such as protein sequencing and functional characterization needs to be done.
要鉴定这些未知功能的的蛋白还需要做进一步的蛋白测序分析及功能鉴定。 - SDS PAGE of whole cell protein of new rhizobial groups and 16S rDNA sequencing of their representatives
根瘤菌新类群的全细胞蛋白电泳及16Srdna全序列分析 - Strategy in Protein Sequencing
蛋白质顺序测定的策略 - When the pET-TYR was transformed into Rosetta ( DE3) the target protein was expressed and identified by amino acid sequencing.
转化到Rosetta（DE3）时，表达出的蛋白经N-末端氨基酸序列测定确定是酪氨酸酶。产物为包涵体，没有活性。 - An Optimized Procedure for Protein and Peptide C-terminal Sequencing by MALDI-TOF Mass Spectrometry
一种优化的MALDI-TOF质谱分析多肽C端序列方法 - Application of liquid chromatography-electrospray ionization mass spectrometry in protein sequencing
液相色谱与串联质谱偶联在蛋白质序列分析中的应用 - The amino acid sequence of the mature protein is identical to α bungarotoxin ( V31), a minor variant of α bungarotoxin identified by protein sequencing technique.
而成熟蛋白的氨基酸序列与从台湾产银环蛇中通过蛋白测序鉴定的α-bungarotoxin的氨基酸序列完全一样。 - Methods Genomic DNA was extracted from the peripheral blood lymphocytes of 22 patients with clinically diagnosed FAP and was forwarded to screening for germline mutations by using denaturing high-performance liquid chromatography ( DHPLC), protein truncation test ( PTT) and DNA sequencing in APC gene.
方法从22例临床确诊的FAP患者，外周静脉血中提取基因组DNA。变性高效液相色谱、蛋白截短检测、测序技术结合应用进行全基因分析。 - With the launch of Human Genome Project and the fast development of Bioinformatics, a great amount of original data concerning protein structure are gained by genome sequencing, protein sequencing and protein structure analysis.
随着人类基因组计划的实施和生物信息学的迅速发展，通过基因组测序、蛋白质序列测定和结构解析等实验，人们获得了大量的关于蛋白质结构的原始数据，并且建立了众多的蛋白质结构数据库。 - Its N-terminal sequence was determined to be N-Met-Arg-Thr-Gln-Glu-His-Thr-Lys-Ser-Val by PE-ABD 491A Protein N-Terminal Sequencing Meter.
用PE-ABD491A蛋白质N-末端测序仪测定其N-末端氨基酸排列顺序是N-Met-Arg-Thr-Gln-Glu-His-Thr-Lys-Ser-Val。 - Molecular Cloning and Sequencing of the Cleavage Site of F0 Protein from the Isolate of Pigeon Newcastle Disease Sequencing Analysis of G Ectodomain off Rabies Vaccine Strain SRV 9
鸽新城疫病毒分离株F0裂解位点的基因克隆及序列分析狂犬病无毒疫苗株SRV9G基因主要功能区的序列分析 - Since transcription regulatory factors are expressed in low level in the cell, it is difficult to obtain enough pure protein for amino acid sequencing or antibody preparation by routine purifying methods.
另外，由于转录调节因子在细胞中的表达水平极低，利用常规的纯化方法很难获得足以进行氨基酸序列分析或抗体制备的纯化蛋白质。 - High precision and automation reagent dispersion presents a wide and important application in the following areas, such as protein isolation, immune analysis, DNA analysis and sequencing, cell culture and detection, and drug screening.
它在蛋白质分离、免疫分析、DNA分析和测序、细胞培养及检测、药物筛选等方面具有重要应用。